WESTERN BLOT
When a western blot was run with the 4T1 BC cells with the CXCR4.1 and CXCR4.2 plasmid inserts, bands appeared at 47 kDa (Fig. 2).
FLOW CYTOMETER
Cells were run through the flow cytometer with and without a stain that binds to CXCR4. 0.754% of cells that did not have the stain in the 4T1 cells fluorescing, while 6.040% of cells with the CXCR4 stain fluoresced (Fig. 3).
FLUORESCENT MICROSCOPE
Post transfection, the cells were observed under a fluorescent microscope. There were cells expressing the GFP fluorescing gene (Fig. 4).
CELL SORTER
0.03% of the 4T1 cells without a plasmid fluoresced while 0.51% of cells with a CXCR4.1 insert fluoresced and 0.25% of cells with a CXCR4.2 insert fluoresced (Fig. 5). These cells were than separated and cultured (Fig. 6).
To see figures, look on figures page.