This science project can be continued by testing the cells with the plasmid inserted into them with a western blot to see if the CXCR4 protein is still being produced in the cells. In the future, an electroporation method will be used as a method for transfection. Electroporation is where electricity is run through the cells to create holes in the cell’s membrane so the circular plasmid can go into the cells. This will hopefully have a higher success rate than the previously used lipid mediated transfection method. From there, if transfection is successful and the gene that codes for CXCR4 is successfully knocked down and there are no CXCR4 proteins being produced in the cancer cells, the experiment can then be moved en vivo in murine models. If this can work in humans, than hopefully, the immune system will no longer be suppressed by MDSCs and be more efficient at killing off cancer cells. This will hopefully make cancer treatments less invasive and more effective.